施工実績

Task out of homologous LGs

LGs that were homologous ranging from F2 and G2F or F2 and you will G2M charts was in fact understood on the basis of a beneficial subset out of 198 and you may 240 common genetics, correspondingly. not, fifteen cases of LG assignment otherwise acquisition inaccuracies was indeed known, suggesting either the existence of paralogous loci (that has been needless to say possible for a couple of markers mapped to various LGs to your G2 and you may F2 charts: AL750495 inside the LG10_G2F and LG8_G2M, and CT577280 from inside the LG7_F2 and you may LG4_G2M) or a great linkage buying condition (which had been the scenario having thirteen non-distorted indicators to provide various other chart cities within the homologous linkage teams (BX678432 inside the LG2_F2 and you can LG2_G2M, CR393801 for the LG4_F2 and you can LG4_G2F, CT580300 in LG4_F2 and you may LG4_G2F, m26 into the LG4_F2 and LG4_G2F, AL749536 inside the LG4_F2 and LG4_G2F, m592 when you look at the LG4_F2 and LG4_G2F, m593 into the LG4_F2 and LG4_G2F, CT577468 into the LG4_F2 and you will LG4_G2F, FN256629 inside the LG4_F and you can LG4_G2M, m738/m739/m740 (exact same contig) when you look at the LG7_F2 and you can LG7_G2M, 384_LIM2 inside LG7_F2 and you will LG7_G2M, BX250169 into the LG7_F2 and LG7_G2M, m590 when you look at the LG7_F2 and you can LG7_G2M). These fifteen family genes have been omitted about range of point indicators. And the anchor markers between F2 and you can G2 charts, 25 testcross indicators (which is, twenty five contigs wherein a few SNPs was indeed polymorphic either in mother or father) were utilized to confirm the brand new homology between LGs on G2F and G2M maps.

Gene thickness

Good Chi 2 test is actually did towards about three maps so you’re able to determine whether the number of genes was uniformly marketed within coastal pine chromosomes. The amount of indicators for every single cM (gene density) are receive in order to differ significantly of a beneficial uniform shipment amongst the 12 linkage groups, within 5% height having G2F and F2 (P-value G2F = 0.012, P-value F2 = 0.00007), and this change was just outside the constraints out-of mathematical advantages having G2M (P-value G2M = 0.074). With the all of the around three maps, there are less family genes from inside the LG 8 and you will more substantial matter off family genes into the LG 6 and you may LG12.

Products affecting recombination

We used the Wilcoxon signed rank test to test the hypothesis that ‘map lengths are equal between the three maps: G2F, G2M and F2.’ This hypothesis was not rejected for the comparison between G2F and G2M, P-value _{(G2F in the place of G2M)} = 0.78, indicating that sex had no significant effect on map length in this mapping population. The same muslim chat room thai test was applied for the comparisons between G2F and F2 and between G2M and F2, with significant differences detected in both cases: P-value _{(F2 as opposed to G2F)} = 0.0004 and P-value _{(F2 instead of G2M)} = 0.005. We checked that the effect of genetic background on the frequency of recombination was not due to the presence of more markers on the F2 map than on the G2F and G2M maps, by carrying out a Wilcoxon signed rank test for all pair-wise recombinations between the common markers in each LG. This test clearly showed that the ‘genetic status’ (intra- versus interprovenance hybrids in our case) of the parental genotypes in which meiotic recombination occurred had a significant effect on the frequency of recombination, with nine LGs presenting significant differences between both F2 and G2F, and F2 and G2M, three LGs presenting a significant difference between F2 and G2F or F2 and G2M, and one LG presenting no effect [see Additional file 8]. Finally, a Z-test was applied to each pair-wise comparison, for the identification of significant pairs among those used to perform the Wilcoxon rank-test (highlighted in red in Figure 3). There was a clear trend toward a greater incidence of significant pair-wise recombination for the F2 map (interprovenance hybrid) than for the G2F or G2M maps (intraprovenance hybrids).